Externally Applied Vibration at 50 Hz Facilitates Dissolution of Blood Clots In-Vitro

¹Ahof Biophysical Systems Inc., 3858 Regent St. Burnaby, B.C., Canada

²In-Vitro Labs , 5407 Canada Way, Burnaby B.C., Canada

*Corresponding Author:

Andrew Hoffmann, B.Sc., Dipl. Tech., RCT(a), RDCS(c)

Ahof Biophysical Systems Inc.

3858 Regent St.

Burnaby, B.C.

Canada, V5C 4G8

Phone (mobile): 1 604 779 3571

Email: andrew.hoffmann11@gmail.com

Abstract

       Background: Localized Low Frequency Vibration (LLFV) in the low sonic range is utilized for disruption of clots by direct contact in catheter applications. However enhanced clot dissolution whereby an LLFV source is applied external from a clotted lumen (such as to resemble a non-invasive therapy) has not been studied.

       Objective: To assess the effectiveness of low amplitude extra luminally applied 50 Hz LLFV in dissolution of 1 hr old clots immersed in Heparinized Saline.

       Methods: One hr old blood clots were each placed within a 3 ml syringe filled with 1.5 ml's of Heparinized Saline. LLFV was then applied against the external surface of each syringe with gentle stroke amplitude (~ 0.5 mm), an intensity within an order of magnitude expected to reach a thrombosed vessel (such as a coronary, pulmonary, cerebral, or peripheral artery), given if a substantially stronger application where to be applied non-invasively across the artery's overlying soft tissue barrier.

       Results: LLFV yielded statistically superior clot dissolution (25%) in comparison to the non vibrated controls (5%) (p<0.0003).

       Conclusions: Transluminally applied LLFV (50 Hz) accelerates clot dissolution in vitro. Further study in this area in-vivo appears warranted.

Keywords: Vibration; Percussion; Thrombosis; Thrombolysis, Acute Myocardial Infarction; Acute Ischemic Stroke; Reperfusion; Emergency Treatment.

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