Investigation of Hibiscus Sabdariffa (Roselle) as Histological Stain and in Assessment of Bar Bodies

¹ Health Services Department, University of Benin, Benin City, Nigeria.

² Department of Medical Laboratory science, Faculty of Health sciences and Technology, Univerisity of Nigeria, Nsukka, Nigeria.

^*Corresponding Author

Omorodion, Nosa Terry

University of Benin

Benin City

Nigeria

Department of Medical Laboratory science

Faculty of Health sciences and Technology

Univerisity of Nigeria

Nsukka

Nigeria

Cell no. +08136742270.

Email: terry.omorodion@uniben.edu

Abstract

A dye is a substance which is used for coloration. Dyes have been in use for ages, nowadays there are several kinds of dyes used in printing, textiles, food, leather and paints industries. They are also used in Histopathology and cytological examination. This study aims at demonstrating specific component of the cells that will be useful in diagnosis. Solution 1 stains Nuclei- dark blue to black, Cytoplasm stains dark brown to pink fibres-dark brown .The structures were weakly demonstrated by Roselle solution 1. Solution 2 stains Nuclei-dark blue to brown, Cytoplasm-golden brown, RBC-brown, Collagen fibres-golden brown. There was good demonstration of those structures. Roselle Solution 3 stains Nuclei-red, Cytoplasm-golden yellow, RBC-brown, Collagen fibres-pale blue. There was optimal demonstration of those structures. Roselle solution 4 stains Nuclei-red, Cytoplasm-golden yellow, RBC-brown, Collagen fibres-golden yellow, Roselle solution 5 stains Nuclei-blue Cytoplasm-pink. It gives the same color as haematoxylin and eosin technique. Buccal smear and blood smear stains with Roselle plant extract and eosin Nuclei-Blue, Cytoplasm-pink, Barr body- not seen. Conclusively, Roselle plant has the ability to stain tissue sections and staining intensity depends on the type of mordant used.

Keywords: Rosellle, Hibiscus sabdariffa Linn, alcohol

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