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Am. J. Biomed. Sci. 2018,10(4),202-210;doi:10.5099/aj180400202
Received:07 October 2018 ; | Revised:09 November 2018; | Accepted: 10 December 2018

 

Stabilizing Ability and Anti-sickling Potentials of Ganoderma Lucidum Decoction Extract on Human HbS Erythrocytes Membrane

 

Reginald Chibueze Ohiri and Eka Bassey Essien

Department of Biochemistry, Faculty of science, University of Port Harcourt. P. M. B. 5323, East-West Road, Choba, Rivers State, Nigeria.

*Corresponding Author

Reginald Chibueze Ohiri

Department of Biochemistry, Faculty of science

University of Port Harcourt. P. M. B. 5323, East-West Road, Choba

Rivers State

Nigeria

Email: raycohiri@yahoo.com, reginald.ohiri@uniport.edu.ng

Tel:+234 8038808077

 

Abstract

Anti-sickling properties of Ganoderma lucidum decoction extract were studied using human HbS erythrocytes. The % lysis, median corpuscular fragility, % stability, polymerisation rate and morphological deformations were determined. The lowest % lysis of (6.76 + 1.63% and 6.15 + 1.96%) were observed in cells treated with 5 mg/ml of extract at 7.0 g/l and 9.0 g/l saline concentrations, while increase in median corpuscular fragility of the erythrocytes were observed, with values of 5.33 + 0.41 g/100g, 5.99 + 0.83 g/100g and 6.08 + 0.51 g/100g for 5 mg/ml, 10 mg/ml and 15 mg/ml of extract. Erythrocytes percentage stability also increased with values of 12.92 + 0.86 %, 26.91 + 1.05 % and 28.81 + 0.94 % for 5 mg/ml, 10 mg/ml and 15 mg/ml of extract, while a pronounced decrease in haemoglobin polymerisation rate was observed at 15 mg/ml of extract from the 2nd to the 3rd minute of analysis with values 0.78 + 0.12 %, 0.47 + 0.07 % and 0.58 + 0.09 % for 2.0, 2.5 and 3.0 minutes. The lowest erythrocytes morphological deformity score was observed at 15.0 mg/ml extract with values of 80.00 + 3.70 %, 56.00 + 2.80 % and 44.00 + 2.20 % at 0.0, 5.0 and 10.0 minutes respectively.

 

Keywords:Ganoderma lucidum, Decoction extract, Erythrocytes, Stability, Polymerization

 

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