Expression Pattern of MALAT-1 Gene and Oxidative DNA Damage in Nigeria Men with Prostate Carcinoma

¹ Department of Medical Laboratory Sciences, School of Basic Medical Sciences, University of Benin, Benin City, Edo State, Nigeria.Email: festusidomeh@gmail.com

² Department of Medical Laboratory Sciences, School of Basic Medical Sciences, University of Benin, Benin City, Edo State, Nigeria.Email: Humphrey.osadolor@uniben.edu

³ Department of Surgery, University of Benin Teaching Hospital, Benin City, Edo State, Nigeria.Email: emmyosa2000@yahoo.com

⁴ Department of Pathology, University of Benin Teaching Hospital, Benin City, Edo State, Nigeria.Email: ikflex@yahoo.com

⁵ Department of Marine Environment and Pollution control, Nigeria Maritime University, Okerenkoko, Delta State. Nigeria.Email: joyceidomeh@gamil.com

^*Corresponding Author

Idomeh Festus Aigbokheo

Department of Medical Laboratory Sciences, School of Basic Medical Sciences, University of Benin

Benin City, Edo State

Nigeria

Email: festusidomeh@gmail.com

Phone Number: +2348074416855

Abstract

Prostate cancer is fast becoming a global burden with negative consequence on the socio-economic status of affected persons around the world. This case control study examined the expression pattern of Metastasis-associated Lung Adenocarcinoma Transcript 1 (MALAT1) gene and levels of 8-Oxoguanine DNA Glycosylase (OGG₁), 8-Hydroxy-2¹-deoxyguanosine (8-OHdG), Cadmium (Cd) and Lead (Pb) in prostate cancer patients of Nigerian origin. A total of fifty-two (52) patients were recruited for this study from the urology clinics of University of Benin Teaching Hospital and Faith Mediplex Hospital, Benin City. They were grouped into A: Prostate cancer patients (CAP), B: Benign prostatic hyperplasia (BPH) and C: apparently healthy participants. Serum blood samples obtained from participants were used to assay for OGG1 and 8-OHdG as markers of DNA damage using ELISA techniques and toxic metals (Cd and Pb) using Inductively Coupled Plasma Mass Spectrophotometer (ICPMS) based on standard methods. Results showed a significant up regulation of MALAT1 gene in CAP and some BPH patients compared to controls (P<0.001). Significantly lower mean levels (P<0.001) of OGG1 (0.84±0.11) were observed for CAP patients when compared with other groups. However, a significantly higher mean level of 8-OHdG (97.24±3.53) (P<0.001), Cd (0.68±0.02) (P=0.003) and Pb (14.46±0.39) (P=0002) was observed for CAP patients when compared with other groups. Our results which showed DNA damage as reflected in lower levels of plasma 8-Oxoguanine DNA Glycosylase and high levels of 8-Hydroxy-2¹-deoxyguanosine, is an evidence of genotoxic insult causing mutation in MALAT-1 gene. Consequently, MALAT-1 gene is up regulated in CAP patients indicating its clinical usefulness as a predictive, diagnosis and prognostic biomarker.

Keywords:Deoxyribonucleic acid damage, Metastasis-associated lung adenocarcinoma transcript 1, Inductively coupled plasma mass spectrophotometer

Download the full article (PDF)