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Am. J. Biomed. Sci. 2020,12(3),155-163;doi:10.5099/aj200300155
Received:24 April 2020; | Revised:10 May 2020; | Accepted:06 September 2020

 

Hepatoprotective Effect of Raphiostylis Beninensis Ethanol Root Extract on Carbon-tetrachloride (CCl4)-induced Liver Attack and Damage in Rats

 

Usunobun U.,1* Osaigbovo J. Ovbokhan and Okolie N. Paulinaus2

1 Department of Biochemistry, Faculty of Basic Medical Sciences, Edo University Iyamho, Edo State, Nigeria

2 Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria

*Corresponding Author

Usunobun Usunomena

Department of Biochemistry,

Faculty of Basic Medical Sciences,College of Medical Sciences

Edo University Iyamho,Edo State

Nigeria

Email: usunobun.usunomena@edouniversity.edu.ng  

Phone: +2348034174871

 

Abstract

This study was conducted to evaluate hepatoprotective effect of ethanol root extract of Raphiostylis beninensis, a plant with several potential therapeutic benefit on Carbon tetrachloride (CCl4)-induced liver damage in rats. Thirty (30) adult male rats weighing 190-200g divided into five (5) groups of six (6) rats each were used. First group received distilled water and served as normal control. Second group received 1ml/kg CCl4 for 4days. Third and fourth group received 1ml/kg CCl4 for 4 days prior to treatment with 150mg/kg and 300mg/kg extract for 8 days respectively. The last group received 1ml/kg CCl4 for 4 days prior to treatment with 100mg/kg Silymarin. Twenty-four hours after last administration, the rats were sacrificed, blood samples and liver tissues obtained for biochemical assays and histology. Biochemical assays showed liver function enzymes/molecules: asparatate aminotransferase (AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP), lactate dehydrogenase(LDH) and total bilirubin to be significantly increased while liver synthetic molecules (total protein and albumin) where decreased significantly in CCl4 treated group when compared to control group whereas treatment with Raphiostylis beninensis caused a significant decrease in liver function enzymes/molecules (AST, ALT, ALP, LDH and total bilirubin) and an increase in liver synthetic molecules (total protein and albumin) when compared to rats which received CCl4 alone. Oxidative stress assessment using superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) and Malondialdehyde (MDA) showed significantly decreased SOD, CAT and GPX and a significantly increase MDA in CCl4-alone group, when compared to control group whereas treatment with Raphiostylis beninensis caused significantly increased SOD, CAT and GPX and a decreased MDA when administered with CCl4 compared to rats which received CCl4 alone. Histopathologically, Raphiostylis beninensis showed reduction in hepatocyte damage, recovery, protection against CCl4 caused degeneration and necrosis, inflammation and nucleus vacuolation.This study showed that Raphiostylis beninensis reduced oxidative stress by decreasing free radical generation due to its enhancement of antioxidant activity.

 

Keywords: Carbon tetrachloride, Free radical, Oxidative stress, Raphiostylis beninensis, Silymarin

 

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