Studies on Antioxidant Role of (+)-Catechin Hydrate in High Sucrose High Fat Diet Induced Oxidative Stress

¹ Department of Biochemistry, Panjab University, Chandigarh-160014, India

² Department of Biophysics, Panjab University, Chandigarh-160014, India

*Corresponding author

Devi Dayal Bansal

Department of Biochemistry

Basic Medical Sciences Block

Panjab University, Chandigarh-160014.

INDIA

Telephone: (O) +91-172-2534131; (M): +919814911579

Email: bansal_devi@yahoo.com

Abstract

In the present investigation, effect of pure green tea catechin, (+)-catechin hydrate was studied on plasma uric acid, plasma nitrite, cardiac lipid peroxidation, reduced glutathione and cardiac antioxidant enzymes in high sucrose high fat diet fed rats for a period of 12 weeks. Our results indicate that feeding high sucrose high fat diet resulted in the development of oxidative stress in rats and (+)-catchin hydrate has antioxidant effect. High sucrose high fat diet decreased plasma uric acid and increased plasma nitrite levels. Further lipid peroxidation increased and reduced glutathione levels decreased in heart of high sucrose high fat diet fed rats. Activity of antioxidant enzymes (SOD, GST, GR and GPx) was significantly reduced in high sucrose high fat fed rats. Catechin supplementation improved plasma uric acid and nitrite levels and reduced lipid peroxidation. Further there was normalization in reduced Glutathione levels and activities of cardiac antioxidant enzymes upon administration of (+)-catechin hydrate. It can be concluded that high sucrose high fat diet produces oxidative stress in rats and administration of catechin is helpful to combat oxidative stress.

Keywords: (+)-catechin hydrate; lipid peroxidation; nitrite; uric acid; glutathione; antioxidant enzymes.

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