Comparative Study for the Therapeutic Effect of Shark Care and 5-Fluorouracil Drugs in Mice with Hepatocelluar Carcinoma in the Presence of Electric Field

Nadia Ahamed Abd El maniem³

¹Medical Biophysics Department, Medical Research Institute, Alexandria University, Alexandria, Egypt

²Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Alexandria, Egypt

³Cancer Management and Research Department, Medical Research Institute, Alexandria University, Alexandria, Egypt

^*Corresponding Author

Rasha Said Shams El-Dine

Medical Biophysics Department

Medical research Institute

Alexandria University

Alexandria 2(03), Egypt

E-mail: Rasha_shams17@yahoo.com

Abstract

Hepatocelluar carcinoma (HCC) is the third deadliest and fifth most common cancer worldwide. Many drugs that have the potential to treat cancers have had limited success due to their lack of efficient and safe delivery mechanisms that allow the drug molecules to cross cell membranes. Electrical pulses-mediated drug delivery, known as electroporation, is gaining attention as a possible approach to enhance uptake of chemotherapy. It delivers anticancer drugs with enhanced efficacy and fewer adverse effects. It is a physical (Thus, applicable to all types of tumors) and is minimally invasive. The aim of the present work was to compare the therapeutic effect of shark care and 5-Fluorouracil drugs in mice with HCC in the presence of electric field. The present study was conducted on 50 albino mice weighting 20-25 gm of 8-10 weeks of age were divided into gp (A) (control), gp (B-1), (That donot recive any treatment), gp (B-2) (which treated by injected by 120 mg/kg of 5-Fluorouracil), gp (B-3) (which treated by shark care drug dissolved in drinking water daily). And after this treatment period part of gp (B-2-i), gp (B-3-i) don’t  exposed to electric field while gp (B-2 –ii) and gp (B-3-ii) were exposed to electric field for 5 second and the last part gp (B-2-iii) and gp (B-3-iii) were exposed to electric field for 10 second. Parts of liver tissue and blood samples were collected from all from mice of each group for histopathological examination, biophysical study (Capacitance and resistance were measured using LCR meter over a freguency range from 1-100KHz and the measured values were used to calculate permittivity and conductivity) and biochemical study (molecular detection of Alpha-fetoprotein (AFP) and transforming growth factor beta-1(TGβ1) mRNAs by RT-PCR) before and after treatment by shark care and 5-Fluorouracil drugs in mice with HCC in the presence of electric field.  During the whole course of experiment the histopathological changes were synchronized with the biophysical (permittivity and conductivity) and biochemical (AFP and TGβ1 mRNAs gene expression) changes. shark care drug showed promising effective results for treatment of HCC compared to results obtained with 5-Fluorouracil drug. presence of electric field results in reduced tumor growth, and induction of apoptosis in the treated cells by enhanceing uptake of chemotherapy and delivering anticancer drugs with enhanced efficacy and fewer adverse effects.

Keywords: 5-Fluorouracil, shark care AFP-mRNA, TGβ1 mRNA, DAB, HCC.

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