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Am. J. Biomed. Sci. Am. J. Biomed. Sci. 2017, 9(1), 20-28; doi: 10.5099/aj170100020 |
Assessment and Comparison of the Skin Irritation Potential of Five Plant Extracts Using In Vitro and In Vivo Methods |
Ning Su1,
Quan An2, Dan Zhao3, Hongyan Zheng1, Li Yang1, Xinshi Li1, Changtao
Wang3,4* |
1Chinese Academy of
Inspection and Quarantine, Daxing District, Beijing,
China |
2Yunnan Baiyao
Group Co., Ltd., Chenggong District, Kunming, China |
3Beijing Key Laboratory of
Plant Resources Research and Development, Beijing Technology and Business
University, Haidian District, Beijing, China |
4Beijing Advanced Innovation
Centre for Food Nutrition and Human Health, Beijing Technology and Business
University, Haidian District, Beijing, China. |
*Corresponding Author |
Changtao Wang |
School of Science |
Beijing Technology and Business University |
Beijing, China |
Tel: 086-010-68984917 |
Email: wangct@th.btbu.edu.cn |
Abstract The aim of this paper was to evaluate the skin irritant potential of five plant extracts for cosmetic use by in vitro and in vivo methods and to analyze the correlation between these different methods. Cell viability in fibroblasts and in the reconstructed human skin model was measured using the MTT assay to test cytotoxicity, and ELISA was used to detect IL-8 release. Finally, human skin patch tests were performed to validate the results obtained using the in vitro methods. The human patch results were compared with IL-8 release results in fibroblasts and the MTT assay findings in EpiskinTM. The MTT assay in fibroblasts showed that the five plant extracts exhibited low cytotoxicity. The human patch results showed that four of the five plant extracts showed weak irritant potential. The MTT assay results in EpiskinTM were best correlated with the human patch test results with a Spearman's correlation coefficient of 0.6156. The Spearman's correlation coefficient between the human patch test and IL-8 release results was 0.4617. The MTT assay in EpiskinTM was more sensitive than the IL-8 release assay in fibroblasts to evaluate skin irritant potential. Our findings indicate that the five plant extracts are safe for cosmetic use. Keywords: Skin
irritation, Fibroblasts, Reconstructed human model, IL-8, Human skin patch test Download the full article (PDF)
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