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Am. J. Biomed. Sci. Am. J. Biomed. Sci. 2017, 9(1), 20-28; doi: 10.5099/aj170100020
Received: 7 December 2016; | Revised: 27 February 2017; | Accepted: 6 March 2017

 

Assessment and Comparison of the Skin Irritation Potential of Five Plant Extracts Using In Vitro and In Vivo Methods

 

Ning Su1, Quan An2, Dan Zhao3, Hongyan Zheng1, Li Yang1, Xinshi Li1, Changtao Wang3,4*

1Chinese Academy of Inspection and Quarantine, Daxing District, Beijing, China

2Yunnan Baiyao Group Co., Ltd., Chenggong District, Kunming, China

3Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Haidian District, Beijing, China

4Beijing Advanced Innovation Centre for Food Nutrition and Human Health, Beijing Technology and Business University, Haidian District, Beijing, China.

*Corresponding Author

Changtao Wang

School of Science

Beijing Technology and Business University

Beijing, China

Tel: 086-010-68984917

Email: wangct@th.btbu.edu.cn

 

Abstract

The aim of this paper was to evaluate the skin irritant potential of five plant extracts for cosmetic use by in vitro and in vivo methods and to analyze the correlation between these different methods. Cell viability in fibroblasts and in the reconstructed human skin model was measured using the MTT assay to test cytotoxicity, and ELISA was used to detect IL-8 release. Finally, human skin patch tests were performed to validate the results obtained using the in vitro methods. The human patch results were compared with IL-8 release results in fibroblasts and the MTT assay findings in EpiskinTM. The MTT assay in fibroblasts showed that the five plant extracts exhibited low cytotoxicity. The human patch results showed that four of the five plant extracts showed weak irritant potential. The MTT assay results in EpiskinTM were best correlated with the human patch test results with a Spearman's correlation coefficient of 0.6156. The Spearman's correlation coefficient between the human patch test and IL-8 release results was 0.4617. The MTT assay in EpiskinTM was more sensitive than the IL-8 release assay in fibroblasts to evaluate skin irritant potential. Our findings indicate that the five plant extracts are safe for cosmetic use.

Keywords: Skin irritation, Fibroblasts, Reconstructed human model, IL-8, Human skin patch test

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