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December 4, 2024 |
Am. J. Biomed. Sci. Am. J. Biomed. Sci. 2017, 9(2), 91-104; doi: 10.5099/aj170200091 |
Hepatitis C Virus Diagnosis in Prospective Blood Donors: Epidemiology, Optimal Testing Approach and Treatment Cut-offs |
Amilo Grace Ifechukwudebelu1, Fasakin Kolawole Asimiyu2*, Ifeanyichukwu Martins Ositadima3 |
1Department
of Haematology, College of Medicine, Nnamdi Azikiwe University
Teaching Hospital, Nnewi, Nigeria |
2Department
of Haematology, Federal Teaching Hospital, Ido Ekiti, Nigeria |
3Department
of Medical Laboratory Science, Faculty of Health Science and Technology, Nnewi Campus, Nnamdi Azikiwe University, Awka, Nigeria |
*Corresponding
Author |
Fasakin Kolawole Asimiyu |
Chief Biomedical Scientist/Lecturer |
Department of Haematology |
Federal Teaching Hospital |
Ido Ekiti |
Nigeria |
Cell no.
+2347031890651 |
Email: fasakin_kolawole@yahoo.co.uk |
Abstract Hepatitis C virus (HCV) infection has been noted a major public health problem with no vaccines available currently. This study aims at optimizing 'safe blood' practice by advancing HCV testing beyond Diaspot rapid enzyme immunoassay in current use in Nigerian Health Institutions. Between August, 2014 and November, 2015, a total of 300 blood donors' plasma samples were screened with both Diaspot and HCV Ag-Ab enzyme-linked immunosorbent assay techniques. HCV-RNA confirmation and quantification were performed with real-time polymerase chain reaction (PCR). Alanine aminotransferase (ALT) was performed on confirmed positive blood donor for treatment purpose. The overall gender ratio and mean age of blood donors screened for HCV were 1.5:1 and 27.67 ± 7.77 years respectively. Of the 300 blood donors screened, 5 (1.67%) and 1 (0.33%) were seropositive for HCV on the basis of Diaspot and enzyme-linked immunosorbent assay (ELISA) techniques respectively. Diagnostic odds ratio showed that ELISA is nearly 9-fold a better diagnostic tool compared to Diaspot technique. Real-time PCR assay confirmed positivity of 1 (0.33%) of the blood donor for hepatitis C. HCV-RNA viral load and plasma ALT of the lone sample were 133, 209 IU/mL and 11.1 IU/L respectively. HCV prevalence among blood donors in Ekiti state is low, 0.33%. Enzyme-linked immunosorbent assay technique should be the starting point of HCV serologic screening and a surrogate technique for real-time PCR. The development of workable algorithm to reduce risks associated with blood transfusion and enhances both blood donors' and recipients' safety is highly imperative. Keywords: Hepatitis C virus, prospective blood
donors, Diagnosis, Diaspot, Real-time PCR Download the full article (PDF)
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