Isolation, Screening and Characterization of Bacteria Having Antibacterial Activity from Industrial Waste Effluent

¹R R Institute of Modern Technology, Bakshi Ka Talab, Sitapur Road, Lucknow

²Faculty of Biotechnology, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Barabanki (UP), India

^*Corresponding Author

Dr. Sunil Kumar

Associate Professor

Faculty of Biotechnology

Institute of Biosciences and Technology

Shri Ramswaroop Memorial University

Lucknow-Deva Road

Vill: Hadauri, Post: Tindola

Dist: Barabanki-225003

Uttar Pradesh (India)

Email: sunil.bio@srmu.ac.in /sunilsbt@gmail.com

Abstract

Background: The aim of the present study was to isolate and screen antibiotic producing bacteria from potential industrial effluents samples of industrial areas mainly Abdullah Tannery Pvt. Ltd (Kanpur), Superhouse Leathers Ltd. (Unnao), UPDPL (Lucknow) and MSF Grow (Kanpur), Uttar Pradesh, India.

Methods: Thirty five soil samples were collected from waste effluent sites, serially diluted and spread on nutrient agar, modified agar-streak and agar-plug methods were used for the primary screening. Cross streak method was used to check antagonistic activity of isolated bacteria against test organisms. Agar well diffusion was used for antimicrobial activity of isolated bacteria against test organisms.

Results: Four isolates (TAAAP010, TAAAP012, TAAAP020 and TAAAP033) have been found to show high antagonistic activity during primary screening. Inhibition zones obtained from agar well diffusion test showed significance differences when compared with standard antibiotics tested against test organisms (P<0.05). Inhibition zone of isolate TAAAP010 against Staphylococcus aureus MTCC 2940, and Bacillus subtilis MTCC 441 were (16±2) mm and (37±1) mm, respectively, which shows greater antimicrobial activity compared to amoxicillin (4 mm). Isolate TAAAP012 showed (22±1) mm and (18±1) mm inhibition zones, respectively. Isolate TAAAP020 has shown inhibition zones of (16±1) mm and (17±1) mm, respectively. However, isolate TAAAP033 has shown high inhibition zones of (28±3) mm and (23±2) mm, respectively.

Conclusion: The research work concluded that the A10 and A33 isolates showed good antibacterial activity against Gram-positive and Gram-negative bacteria. The A10 and A33 isolates were confirmed as Bacillus subtilis and Streptomyces griseus by 16S rRNA studies.

Keywords: Antimicrobial metabolites, industrial effluent, antagonism, enzymes, soil

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