Antimicrobial Activities of Chrysophyllum Albidum Leaves, Fruits and Seeds

1 Medical Laboratory Science Council of Nigeria, South-South Zonal office, Benin city, Nigeria

2 Department of Medical Laboratory Science, College of Medicine, University of Lagos, Nigeria

3,4 Public Health In-vitro Diagnostic Control Laboratory, Medical Laboratory Science Council of Nigeria, Yaba-Lagos, Nigeria.

^*Corresponding Author

George, Akintunde Olajide

Medical Laboratory Science Council of Nigeria

73, Murtala Mohammed Way, Benin City, 

Edo State,

Nigeria.

E-mail: georgeolajide2016@gmail.com

Cell phone: +2348062234415

Abstract

Background: Chrysophyllum  albidum is a medicinal plant that belongs to the Sapotaceae family. This study was carried out to determine the antimicrobial activities of the leaves, seeds and fruits of Chrysophyllum albidum.

Materials and Methods: The fruits, leaves and seeds of Chrysophyllum albidum were extracted with distilled water, Seaman’s Schnapps, Methanol and Petroleum ether using cold extraction. These extracts were tested on various organisms. Antimicrobial activities of the various extracts were determined using the agar well diffusion technique to detect the Minimum Inhibitory Concentration (MIC).

Results and Discussion: Methicillin Resistant Staphylococcus aureus was inhibited by all the extracts at MIC between 32µg/ml to 512µg/ml except Seaman’s Schnapps leaves and petroleum ether seed extract. The fruit extracts had a high antimicrobial activity against all the tested organisms. The extracts from Seaman’s Schnapps had little activity while the aqueous extracts had MIC ranging from 64- 512µg/ml on most of the organisms tested. The statistical analysis of the extracts indicated P-value of 0.122 for Aqueous extracts, which was statistically insignificant compared to 0.003 for Methanolic extracts and 0.001 for Seaman’s Schnapps extracts in the inhibition pattern of the extracts. Aqueous extracts showed more inhibitory activity than Methanolic and Seaman’s Schnaps extracts.

Conclusion: Chrysophyllum albidum possess antimicrobial properties that can also be developed as antimicrobial for Multidrug resistant (MDR) organisms. Chyrsophyllum albidum is therefore recommended to be developed into new antimicrobials in readily available forms for use in Medical Microbiology Laboratory.

Keywords: Chrysophyllum albidum;Antimicrobial; MIC;Extracts.

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