Effect of Obestatin on Gastric Acid Secretion and Mucin Expression in Normal and Streptozoocin Induced Diabetic Rats

Physiology Department, Faculty of Medicine, Zagazig University, Egypt

^*Corresponding Author

Dr. Reham Hassan Ibrahim

Physiology Department, Faculty of Medicine

Zagazig University

Egypt

Email: phisiology_lover_4@yahoo.com

Abstract

Background: Obestatin is a peptide hormone derived from the same peptide precursor as ghrelin, it regulates food intake and gastric motility, however, to date, no studies conducted about the effects of obestatin on gastric acid secretion and gastric mucin expression. Aim: investigate effects of obestatin on gastric acid secretion and mucin expression in normal and streptozoocin (STZ) induced diabetic male rats. Material and Methods: 32 adult male albino rats were divided into group I (normal) and group II STZ induced diabetic rats, each group further subdivided into two equal subgroups: group a (received intra-peritoneal (ip) saline  daily for 21 days) and group b (daily received ip obestatin "10 nmol/kg" for 21 days). In all groups, gastric secretion was collected, then total acid output, ghrelin and volume of gastric secretion were measured, real time polymerase chain reaction (PCR) for gastric mucin (MUC5AC) and gastric H+-K+-ATPase α-subunit  gene expression were measured, blood samples were obtained for insulin and glucose measurement. Results: in both normal and diabetic rats, obestatin increased total acid output, ghrelin, volume of gastric secretion, gastric H+-K+-ATPase α-subunit and MUC5AC gene expression. There was none significant change in blood glucose and insulin in normal rats, while significant increase in insulin level and significant decrease in blood glucose in STZ induced diabetic rats. Conclusion: obestatin on chronic run increased gastric acidity, ghrelin and volume of gastric secretion in normal and diabetic rats, obestatin can be considered as gastro- protective agent as it increased gastric mucin in normal and diabetic rats.

Keywords:Obestatin, Gastric secretion, Acid, Diabetes, Ghrelin, H+-K+-ATPaset

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