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Am. J. Biomed. Sci. 2019, 11(2), 65-73;doi:10.5099/aj190200065
Received:01 January 2019 ; | Revised:07 January 2019; | Accepted: 04 June 2019

 

Bacteriological Identification among Patients Attending Intensive Care Unit at Nemba District Hospital 

 

Callixte Yadufashije1, Thierry Habyarimana1, John Karemera2, Cedrick Izere2,

Monique Mukundente3, Ange Yvette Uwitonze2

1PhD, Department of Biomedical Laboratory sciences, INES-Ruhengeri, Rwanda

2MSc, Department of Biomedical Laboratory sciences, INES-Ruhengeri, Rwanda

3BSc, Department of Biomedical Laboratory sciences, INES-Ruhengeri, Rwanda

*Corresponding Author

Dr. Callixte Yadufashije

Department of Biomedical Laboratory

INES-Ruhengeri

Rwanda

Email: cyadufashije@ines.ac.rw, cyadufashije@gmail.com

Vietnam

 

Abstract

Background: This study entitled bacteriological identification among patients attending intensive care unit at Nemba District Hospital was conducted in bacteriology unit of Nemba District Hospital. The range between 5% and 15% of hospital patients in an intensive care unit is 5-10 times more likely acquiring a nosocomial infection.

Objectives: The study was objectively assessed bacterial infections among patients attending ICU and predominant bacteria were mentioned. Both sensitivity and resistance of antibiotics were performed to know the better treatment for infections in ICU patients.

Methods: The samples were collected from intensive care unit services and phlebotomy of Nemba District Hospital and tested in laboratory in the service of bacteriology unit and bacteria were identified by means of a gram stain, biochemical tests and culture.

Results and conclusion: Findings of conducted study showed that the predominant bacteria in intensive care unit. Among predominant bacteria identified include Staphylococcus Aureus (37%), Escherichia coli (24%), Salmonella typhi (35%), Pseudomonas aeruginosa (1%) and Klebsiella pneumoniae (3%) Among the antibiotic susceptibility test, Staphylococcus Aureus showed the total resistant on penicillin while tetracycline showed to have the highest sensitivity. Salmonella typhi showed resistance on amoxicillin (100%), Escherichia coli showed the total resistant on gentamicin (100%).

Hospitalized patients in intensive care unit are at risk of acquiring Nosocomial infections due to the presence of mentioned bacteria during this study. The big issue is that these infections resist to antibiotics used in treatment according to results of the study. Health care workers in ICU should improve hygiene to control hospital acquired infections and much attention should be done during diagnosis to improve treatment of patients.

 

Keywords:Bacteria, Antibiotics, Infection, Patient

 

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